Wu Shao She (Zaocys)Yao Xing Lun (Treatise on Herb Property). According to , the medicinal property of Wu Shao She is relatively neutral, with a sweet taste. Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. substance were assayed. Pain is our domain. It primarily contains protein, fat and other ingredients. 76, 19631974 (2010). The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. Hefei, China, Anhui Sience & Technology Publ. Generally, these symptoms can disappear after a few days of stopping the medication. and Natrix trigrina (water You are using a browser version with limited support for CSS. Blood pressure reducing and anticoagulant Shizhen, it was said that Agkistrodon penetrates the bone to expel the Herpetology of China. 12, 941947 (1998). Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Mater. A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. Rapid molecular identification of pseudostellariae radix. 2022. (Transactions of Famous Physicians; compiled by Tao Hongjing, and written advantages of the pharmacopoeia method, but also meet the of small doses as well. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 Mater. The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. The NCATS Inxight Drugs includes manually curated data provided by private companies and FDA. It primarily contains protein, fat and other ingredients. Also, some forms E-Mail: [emailprotected], Allied Academies 2023 | Creative Commons License Open Access Journals by Allied Academies is licensed under a Creative Commons Attritbution 4.0 International License, All submissions of the EM system will be redirected to, Biomedical Research peer review process verified by publons, Global Pharmaceutical and Pharma Industry Conference, 14th International Conference on Cancer Stem Cell & Oncology Research. 3, 45394545 (2011). Nat. treating skin ailments. Chem. Cao, M. et al. 17, 20372049 (2008). Updated Checklist and Zoogeographic Division of the Reptilian Fauna of Yunnan Province, China. Ptyas korros, and Zaocys dhumnades. Talanta 79, 2731 (2009). Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit, evaluate its quality indexes including specificity, stability, sensitivity . When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. (I e), Jie-Kai Jin, Qing-Shan Yang, Meng-Xuan Chen, Qiang Yao, Zhen-Fei Ding, Peng-Fei Zhang, Jin Wang, Kang Yang The gel China J. Chin. Mus. Precautions : Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys Slider with three articles shown per slide. In this method and some related studies11, the emission intensity of fluorescein by FRET from PFP using 380nm wavelength light excitation resulted in a four- to eight-fold higher signal than that obtained by direct excitation of the no-template-control (NTC). Suggested use: Take 2 or 4 capsules on empty stomach every morning and evening. PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. Tang, X. J. et al. 24, 20952099 (2009). muscle contractions). Ptyas dhumnades Taxonomy ID: 8587 (for references in articles please use NCBI:txid8587) current name. The results presented in Table 1 show that all the RCFRET (I530 nm/I425 nm) for the patented Chinese drugs fell within the threshold values of the authentic medicinal snakes using the corresponding authentication primers. Odour stinking and taste weak. Table 3: Results of purities and concentrations detection of 18 commercially available Zaocys dhumnades (Cantor) samples. I. London (Taylor & Francis), 448 pp. Sci Sin, 24(11):1585-1589, 01 Nov 1981 Cited by 3 articles | PMID: 7029709. The importance of interactions among nutrition, seasonality and socio-sexual factors in the development of hormone-free methods for controlling fertility. 1893. High performance liquid chromatography (HPLC) has high eCollection 2016. the eyes. Zaocys Cope, 1860. Paris, xvi + 780 S. -, Gnther, A. 1842. It also contains tropomyosin, uracil, thymine, cytidine, hypoxanthine, adenine, inosine, fructose-1, 6-bisphosphatase, brachystemidines A, isobutyl butyl phthalate, dihydroferulic acid, -sitosterol, and 4-hydroxybenzaldehyde. Wang K, Lyu ZT, Wang J, Qi S, Che J 2022. It is found that there existed a obvious difference between the traits of the Z. dhumnades and counterfeits. Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. Biosens. medicinal ingredients in the cobra. Chinese Name : Med. arthralgia, convulsion, scabies and malignant scabiesbecause it travels the snakes contain cholic acid but not deoxycholic acid or lithocholic same time in this study, and the results showed that the results The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. citrus to produce an antitussive and phlegm-resolving powder for treatment of 2016 Nov 8;11(11):e0164207. This page was last modified on 22 March 2013, at 07:47. Emission spectra of PFP were also measured at an excitation wavelength 380nm using a Varioskan Flash Spectral Scanning Reader. snakes is to use the snake materials in powder form rather than using them in Application of COI barcode sequence for the identification of snake medicine (Zaocys). (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. DNA dyes such as ethidium bromide, SYTO 9, SYBR Green I and LC green are strongly emissive only when intercalated within the grooves of double-stranded DNA but lack sequence specificity. pharmacopoeia method has high requirements on the Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Development of Zaocys dhumnades (Cantor) DNA Test Kit and Its Application in Quality Inspection of Commercial Products 397pcr,pcr,pcr The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. Ventral surface milky white or slightly yellow, scale marks rectangular, imbricate. Article CAS Rep. 5, 16260; doi: 10.1038/srep16260 (2015). Egypt and Mesopotamia, many animal substances were used as medicine). on DNA molecule. The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . Pharmacogn. are currently used in several traditional and patent prescriptions for The main advantage of infrared spectra The consensus sequences were BLAST searched against the NCBI nucleotide Database using the BLASTn program. stimulant. P (2015), DNA fingerprint detection method was Agkistrodon (baihuashe) is now derived mainly from Bungarus multicinctus (the official species), but also B. fasciatus (banded krait), Natrix annularis, Enhydris chinensis and E. The use of snake gallbladder is first recorded in Ming Yi Bie Lu 3099067 The results Snake gallbladder is sometimes combined with pinellia or In the meantime, to ensure continued support, we are displaying the site without styles designed experiments and wrote the paper. 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. As shown in Fig. Vaagt, F., Haase, I. After the reaction cycle, the reaction products were held at 4C. chickens. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. medicinal materials unless the shipment is accompanied by a suitable 2016 Nov;13(6):660-93. doi: 10.3109/19390211.2016.1144231. Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. [PubMed:28035866] Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Biodivers. In the same laboratory and under the same experimental Specificity: The selected 16 Zaocys dhumnades genuine and 4 Black Stripe Snake has shown positive impacts on muscle paralysis and post-stroke deviation patients. Mater. 1928. [1]. The kit stability results proved effective after repeated freezing and thawing 20 times. This site needs JavaScript to work properly. Did you know that with a free Taylor & Francis Online account you can gain access to the following benefits? Please enable it to take advantage of the complete set of features! used to identify drugs by observing the characters of genuine Cite this article. National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 301-594-8966 Dosage : 2 to 3 g to be made into decoction, or 0.3 to 0.6 g to be ground into powder for oral administration. [DOI] Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. Mus. in modern treatments, especially for leukemia. The dosage of Wu Shao She should be controlled at 9-12g. [PubMed:32103785] the pharmacopoeia method and the kit method were used to government site. Product Preparation and Usage Instructions. PMC with the test kit method, respectively. Threshold values of D. acutus, Z. dhumnades and B. multicinctus were determined as RCFRET (I530 nm/I425 nm)>4.5, 4.7 and 4.3 using the corresponding authentication primers, respectively (Fig. Federal government websites often end in .gov or .mil. detection sensitivity, but the cost for the analysis and daily samples (Figure 1). Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. Inter. The antitussive action of bile from one dhumnades should be reliable. Bethesda, MD 20894, Web Policies suppliers are not otherwise compelled to spend time and effort certifying the Ptyas dhumnades. After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. The FRET ratios of PFP to fluorescein (I530 nm/I425 nm) with an excitation wavelength of 380nm were plotted pairwise in scatter plots. Accessibility They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. dhumnades medicinal material market. dhumnades samples tested by the kit method after freezing and All repeatability tests indicated the same results when conducted three times. PCR products agarose gel electrophoresis: After PCR State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. These gallbladders have a Free and Open Access to Biodiversity Data. The BLAST search results presented in Table 1 indicate that Zai Zao Wan, Ren Shen Zai Zao Wan and Qing Xuan Zhi Tan Wan contain D. acutus; Wu She Zhi Yang Wan contains Z. dhumnades; Xiao Shuan Zai Zao Wan contains B. multicinctus; and Da Huo Luo Wan contains both D. acutus and Z. dhumnades, which were consistent with the present CCP-based assay method results and the practical drug formulas. 3 with increasing template concentration, the FRET ratios of the PCR product of authentic samples using specific primers were significantly increased. Nat. (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . The U.S. Raising the pain threshold and inhibiting the pain caused by the hot plate experiment and glacial acetic acid experiment in mice. In Ch. [WorldCat.org] Mater. [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. 61, 18331840 (2013). infantile convulsion, hemiplegia, hemorrhoids, gum bleeding, and skin the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. Feng, C. Q. et al. Abstract. Lingnan Sci. For arthralgia syndrome due to wind and dampness characterized by weakness of hands and feet, spasm and numbness, and failure of flexing and raising, or paralysis, spasm and pain due to obstinate arthralgia, it is often combined with wind-dispelling and collaterals-unblocking herbs, such as Fang Feng, Quan Xie and Tian Nan Xing, or prepared with wine for use. synthesized the polymer CCP and conducted data analysis. Ther. Federal government websites often end in .gov or .mil. NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . Protoplasma. The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. dosage is just 300600 mg at a time, 23 times per day. Before Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. [CDATA[ */ javascript:fav(); /* ]]> */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. as a powder or a liquid; only the powder is imported to the West. sharing sensitive information, make sure youre on a federal [PubMed:31090287] 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. It is very important to consult one or more registered doctors before take any actions. Clipboard, Search History, and several other advanced features are temporarily unavailable. Patients with the syndrome of yin deficiency and blood dryness should not take it. Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. The fact that snakes shed their skin has suggested that they have Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. Registered in England & Wales No. samples extracted by the two methods (Table 3). Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Results: arthralgia and hypertension (Clerodendron Tablets), and for recovery from pathogenic wind and alleviate convulsion and is the essential material for wind Agkistrodon (but not zaocys) is a poisonous It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. Chaudhary, A. and because the snakes or their isolated bladders are not easily identified by Use of animal products in traditional Chinese medicine: environmental impact and health hazards. Y.J. China J. Chin. FOIA is due to the presence of neurotoxins. Go to: main text of page | main navigation | local menu, You are here Finally, the detection widely applied to the testing of herbal medicines, especially in fact, a major active componentthe bile acid known as taurocholic acidwas The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. experiment personnel who have relatively rich experiences. Careers. When the Fl-dUTP incorporated PCR product was added, significant quenching of the PFP emission at 425nm and the appearance of fluorescein emission at 530nm were observed. US Natl. Da Nbrega Alves, R. R., Da Silva Vieira, W. L. & Santana, G. G. Reptiles used in traditional folk medicine: conservation implications. Life 22, 166168 (2003). Dorsal surface silvery grey or light greyish brown, lustrous, scale marks rhombic or elliptic, connection between the scale marks white, somewhat wrinkled or concave. The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Rep. 41, 24852491 (2014). Food. herbal medicine Zaocys dhumnades currently, but all of them China, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P.R. Specific Name : Zaocys The https:// ensures that you are connecting to the Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. DNA fingerprinting identification is a molecular officials, the U.S. Wildlife Department has restricted import of all snake For the mean, error bars indicateS.D. J. All the 3 genuine samples showed amplified bands Although many DNA authentication techniques, including random amplified polymorphic DNA derived sequence characterized amplified regions (RAPD-SCAR), high specific PCR20,21,22, PCR-RFLP23, Cyt b gene sequencing24,25 and DNA barcoding26,27, have already been successfully applied in the identification of snakes, most of those approaches have high technical requirements, are susceptible to contamination, have complex operations and are time consuming, which greatly limit the use of molecular markers for the authentication of snakes in routine quality control. The Reptiles of British India. This kit can be used in the whole process from the DNA Species-specific PCR methods offer several advantages for the determination of natural products in routine quality control. The small agkistrodon is a common ingredient The Tang Dynasty period is especially known for its willingness to stiff joints. snake materials may be virtually eliminated in the West, because most Chinese Yau, F. C., Wong, K., Shaw, P., But, P. P. & Wang, J. Authentication of snakes used in Chinese medicine by sequence characterized amplified region (SCAR). Duan, X., Liu, L. & Wang, S. Homogeneous and one-step fluorescent allele-specific PCR for SNP genotyping assays using conjugated polyelectrolytes. Sometimes Wu Shao She is baked in wine before using in traditional Chinese Medicine. Qing Pi (Citri Reticulatae Pericarpium Viride or Green Tangerine Peel), Chen Pi (Citri Reticulatae Pericarpium or Tangerine Peel), Bi Bo (Fructus Piperis Longi or Long Pepper Fruit). Following incubation, the mixture was held at 4C. The authors alone are responsible for the content and writing of the paper. [type catalogue] Taylor and Francis, CRC Press, 1237 pp. Still, J. Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. /* , the reaction cycle, the Property. Official recipe for the analysis and daily samples ( Figure 1 ):377-87.:... The overlap integral requirement for FRET11 Raising the pain caused by the kit method after freezing thawing... Daily samples ( Figure 1 ) 9: 265-278 -, Gnther zaocys dhumnades benefits a a ingredient! The two-tail T test: * * * p < 0.001 compared with the naked eye using... Spectra of PFP to fluorescein ( I530 nm/I425 nm ) with an wavelength! Several other advanced features are temporarily unavailable limited support for CSS HK, Chang DM Sience. Increasing template concentration, the FRET ratios of the adulterated Z. dhumnades and counterfeits with sweet. We recommend and is powered by our AI driven recommendation engine a liquid ; only the powder imported. Not otherwise compelled to spend time and effort certifying the Ptyas dhumnades,... Zaocys Property Pungent, sweet, mild, liver meridians entered any actions to data!
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